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adenoviral infection  (Vector Biolabs)


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    Vector Biolabs adenoviral infection
    Transcriptional regulation of LIN28A and LIN28B by developmental transcription factors. A) Schematic of transcription factors that orchestrate germ layer formation and early embryonic lineage specification and differentiation. B) Top: Schematic of morphogen-induced lung/endoderm formation from iPSCs through initiation of lung progenitors in vitro. Bottom: Gene expression analysis of single cell RNA-sequencing of roughly 10,000 cells (close 1200 for each day) throughout morphogen-directed lung endoderm formation from iPSCs. Data from manuscript (Ori et al., 2023). C) Fold change in gene expression of LIN28A and LIN28B upon infection of embryonic lung fibroblast WI-38, with <t>adenoviral</t> constructs: GFP, FOXA2, NKX2-1, SOX2 and SOX9 for 48–72 h before lysing in Trizol. Dots on the graph representative of n = 2 independent experiments in quadruplicate. D) Schematic of development of endoderm differentiation. Transient transfection of txrn factors into HEK293 with construct stably expressing dual luciferase construct; firefly and Renilla luciferase. Validation of a subset of factors that regulated human LIN28A and LIN28B promoter/enhancer sequences during screening experiments in panels B) and C) during E) pluripotency and F) endoderm/lung development. G) progenitor specification. Graphs in E), F) and G) are representative of n = 2 independent validation experiments out of 3. P values for C) were determined using 2-way ANOVA of normal distribution. P values of significance: In the order of left to right for panel C: LIN28A – FOXA2- ∗0.024, NKX2-1- ∗0.035, SOX9- ∗0.021; LIN28B – NKX2-1- ∗0.021, ∗SOX9- 0.021 P values for graphs E), F) and G) were determined using 1-way ANOVA of normal distribution in E) and 1-way ANOVA of lognormal distribution for F) and G). P values of significance: In the order of left to right for each panel: E)∗ 0.0375, ∗∗ 0.0021; ∗ 0.0184, ∗0.0321 F)∗ 0.0434, ∗0.0325; ∗0.0191, ∗0.0477 G)∗∗0.0097,∗ 0.0112; ∗∗0.0021, ∗∗0.0078; ∗∗ 0.0036, ∗∗0.0079; ∗∗0.0072, ∗0.0160. Schematics A), B), D) were made using BioRender.
    Adenoviral Infection, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/adenoviral+infection/pmc12465051-263-7-12?v=Vector+Biolabs
    Average 96 stars, based on 162 article reviews
    adenoviral infection - by Bioz Stars, 2026-07
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    Images

    1) Product Images from "LIN28-mediated gene regulatory loops synchronize transitions throughout organogenesis"

    Article Title: LIN28-mediated gene regulatory loops synchronize transitions throughout organogenesis

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2025.102226

    Transcriptional regulation of LIN28A and LIN28B by developmental transcription factors. A) Schematic of transcription factors that orchestrate germ layer formation and early embryonic lineage specification and differentiation. B) Top: Schematic of morphogen-induced lung/endoderm formation from iPSCs through initiation of lung progenitors in vitro. Bottom: Gene expression analysis of single cell RNA-sequencing of roughly 10,000 cells (close 1200 for each day) throughout morphogen-directed lung endoderm formation from iPSCs. Data from manuscript (Ori et al., 2023). C) Fold change in gene expression of LIN28A and LIN28B upon infection of embryonic lung fibroblast WI-38, with adenoviral constructs: GFP, FOXA2, NKX2-1, SOX2 and SOX9 for 48–72 h before lysing in Trizol. Dots on the graph representative of n = 2 independent experiments in quadruplicate. D) Schematic of development of endoderm differentiation. Transient transfection of txrn factors into HEK293 with construct stably expressing dual luciferase construct; firefly and Renilla luciferase. Validation of a subset of factors that regulated human LIN28A and LIN28B promoter/enhancer sequences during screening experiments in panels B) and C) during E) pluripotency and F) endoderm/lung development. G) progenitor specification. Graphs in E), F) and G) are representative of n = 2 independent validation experiments out of 3. P values for C) were determined using 2-way ANOVA of normal distribution. P values of significance: In the order of left to right for panel C: LIN28A – FOXA2- ∗0.024, NKX2-1- ∗0.035, SOX9- ∗0.021; LIN28B – NKX2-1- ∗0.021, ∗SOX9- 0.021 P values for graphs E), F) and G) were determined using 1-way ANOVA of normal distribution in E) and 1-way ANOVA of lognormal distribution for F) and G). P values of significance: In the order of left to right for each panel: E)∗ 0.0375, ∗∗ 0.0021; ∗ 0.0184, ∗0.0321 F)∗ 0.0434, ∗0.0325; ∗0.0191, ∗0.0477 G)∗∗0.0097,∗ 0.0112; ∗∗0.0021, ∗∗0.0078; ∗∗ 0.0036, ∗∗0.0079; ∗∗0.0072, ∗0.0160. Schematics A), B), D) were made using BioRender.
    Figure Legend Snippet: Transcriptional regulation of LIN28A and LIN28B by developmental transcription factors. A) Schematic of transcription factors that orchestrate germ layer formation and early embryonic lineage specification and differentiation. B) Top: Schematic of morphogen-induced lung/endoderm formation from iPSCs through initiation of lung progenitors in vitro. Bottom: Gene expression analysis of single cell RNA-sequencing of roughly 10,000 cells (close 1200 for each day) throughout morphogen-directed lung endoderm formation from iPSCs. Data from manuscript (Ori et al., 2023). C) Fold change in gene expression of LIN28A and LIN28B upon infection of embryonic lung fibroblast WI-38, with adenoviral constructs: GFP, FOXA2, NKX2-1, SOX2 and SOX9 for 48–72 h before lysing in Trizol. Dots on the graph representative of n = 2 independent experiments in quadruplicate. D) Schematic of development of endoderm differentiation. Transient transfection of txrn factors into HEK293 with construct stably expressing dual luciferase construct; firefly and Renilla luciferase. Validation of a subset of factors that regulated human LIN28A and LIN28B promoter/enhancer sequences during screening experiments in panels B) and C) during E) pluripotency and F) endoderm/lung development. G) progenitor specification. Graphs in E), F) and G) are representative of n = 2 independent validation experiments out of 3. P values for C) were determined using 2-way ANOVA of normal distribution. P values of significance: In the order of left to right for panel C: LIN28A – FOXA2- ∗0.024, NKX2-1- ∗0.035, SOX9- ∗0.021; LIN28B – NKX2-1- ∗0.021, ∗SOX9- 0.021 P values for graphs E), F) and G) were determined using 1-way ANOVA of normal distribution in E) and 1-way ANOVA of lognormal distribution for F) and G). P values of significance: In the order of left to right for each panel: E)∗ 0.0375, ∗∗ 0.0021; ∗ 0.0184, ∗0.0321 F)∗ 0.0434, ∗0.0325; ∗0.0191, ∗0.0477 G)∗∗0.0097,∗ 0.0112; ∗∗0.0021, ∗∗0.0078; ∗∗ 0.0036, ∗∗0.0079; ∗∗0.0072, ∗0.0160. Schematics A), B), D) were made using BioRender.

    Techniques Used: In Vitro, Gene Expression, RNA Sequencing, Infection, Construct, Transfection, Stable Transfection, Expressing, Luciferase, Biomarker Discovery



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    Transcriptional regulation of LIN28A and LIN28B by developmental transcription factors. A) Schematic of transcription factors that orchestrate germ layer formation and early embryonic lineage specification and differentiation. B) Top: Schematic of morphogen-induced lung/endoderm formation from iPSCs through initiation of lung progenitors in vitro. Bottom: Gene expression analysis of single cell RNA-sequencing of roughly 10,000 cells (close 1200 for each day) throughout morphogen-directed lung endoderm formation from iPSCs. Data from manuscript (Ori et al., 2023). C) Fold change in gene expression of LIN28A and LIN28B upon infection of embryonic lung fibroblast WI-38, with adenoviral constructs: GFP, FOXA2, NKX2-1, SOX2 and SOX9 for 48–72 h before lysing in Trizol. Dots on the graph representative of n = 2 independent experiments in quadruplicate. D) Schematic of development of endoderm differentiation. Transient transfection of txrn factors into HEK293 with construct stably expressing dual luciferase construct; firefly and Renilla luciferase. Validation of a subset of factors that regulated human LIN28A and LIN28B promoter/enhancer sequences during screening experiments in panels B) and C) during E) pluripotency and F) endoderm/lung development. G) progenitor specification. Graphs in E), F) and G) are representative of n = 2 independent validation experiments out of 3. P values for C) were determined using 2-way ANOVA of normal distribution. P values of significance: In the order of left to right for panel C: LIN28A – FOXA2- ∗0.024, NKX2-1- ∗0.035, SOX9- ∗0.021; LIN28B – NKX2-1- ∗0.021, ∗SOX9- 0.021 P values for graphs E), F) and G) were determined using 1-way ANOVA of normal distribution in E) and 1-way ANOVA of lognormal distribution for F) and G). P values of significance: In the order of left to right for each panel: E)∗ 0.0375, ∗∗ 0.0021; ∗ 0.0184, ∗0.0321 F)∗ 0.0434, ∗0.0325; ∗0.0191, ∗0.0477 G)∗∗0.0097,∗ 0.0112; ∗∗0.0021, ∗∗0.0078; ∗∗ 0.0036, ∗∗0.0079; ∗∗0.0072, ∗0.0160. Schematics A), B), D) were made using BioRender.

    Journal: Biochemistry and Biophysics Reports

    Article Title: LIN28-mediated gene regulatory loops synchronize transitions throughout organogenesis

    doi: 10.1016/j.bbrep.2025.102226

    Figure Lengend Snippet: Transcriptional regulation of LIN28A and LIN28B by developmental transcription factors. A) Schematic of transcription factors that orchestrate germ layer formation and early embryonic lineage specification and differentiation. B) Top: Schematic of morphogen-induced lung/endoderm formation from iPSCs through initiation of lung progenitors in vitro. Bottom: Gene expression analysis of single cell RNA-sequencing of roughly 10,000 cells (close 1200 for each day) throughout morphogen-directed lung endoderm formation from iPSCs. Data from manuscript (Ori et al., 2023). C) Fold change in gene expression of LIN28A and LIN28B upon infection of embryonic lung fibroblast WI-38, with adenoviral constructs: GFP, FOXA2, NKX2-1, SOX2 and SOX9 for 48–72 h before lysing in Trizol. Dots on the graph representative of n = 2 independent experiments in quadruplicate. D) Schematic of development of endoderm differentiation. Transient transfection of txrn factors into HEK293 with construct stably expressing dual luciferase construct; firefly and Renilla luciferase. Validation of a subset of factors that regulated human LIN28A and LIN28B promoter/enhancer sequences during screening experiments in panels B) and C) during E) pluripotency and F) endoderm/lung development. G) progenitor specification. Graphs in E), F) and G) are representative of n = 2 independent validation experiments out of 3. P values for C) were determined using 2-way ANOVA of normal distribution. P values of significance: In the order of left to right for panel C: LIN28A – FOXA2- ∗0.024, NKX2-1- ∗0.035, SOX9- ∗0.021; LIN28B – NKX2-1- ∗0.021, ∗SOX9- 0.021 P values for graphs E), F) and G) were determined using 1-way ANOVA of normal distribution in E) and 1-way ANOVA of lognormal distribution for F) and G). P values of significance: In the order of left to right for each panel: E)∗ 0.0375, ∗∗ 0.0021; ∗ 0.0184, ∗0.0321 F)∗ 0.0434, ∗0.0325; ∗0.0191, ∗0.0477 G)∗∗0.0097,∗ 0.0112; ∗∗0.0021, ∗∗0.0078; ∗∗ 0.0036, ∗∗0.0079; ∗∗0.0072, ∗0.0160. Schematics A), B), D) were made using BioRender.

    Article Snippet: Lin28a/b were knocked out post-neurosphere formation via adenoviral infection with Ad-Cre-GFP adenovirus (Vector Biolabs, 1700) or Ad-GFP adenovirus (Vector Biolabs, 1060).

    Techniques: In Vitro, Gene Expression, RNA Sequencing, Infection, Construct, Transfection, Stable Transfection, Expressing, Luciferase, Biomarker Discovery